Artemisinin Inhibits the Migration and Invasion in Uveal Melanoma via Inhibition of the PI3K/AKT/mTOR Signaling Pathway

Figure 5

Artemisinin inhibited PI3K/AKT/mTOR signaling pathway in UM cells. (a, e, h) UM cells were treated with different doses of artemisinin, and Western blot analysis was performed to detect p-PI3K, PI3K, p-AKT, AKT, p-mTOR, mTOR, E-cadherin, N-cadherin, Vimentin, Bcl2, and cleaved caspase 3 expression. (e) Artemisinin altered the expression levels of EMT markers in UM cells. (h) Artemisinin decreased the protein expression of Bcl2 and increased the protein expression of cleaved caspase 3, suggesting an increase in apoptosis. β-Actin and β-tubulin were used as loading controls. (b–d, f, g, i, j) Quantification of the bands in (a). All the results represent the of three independent experiments versus the control group. were considered statistically significant.

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