Resveratrol Prevents Right Ventricle Dysfunction, Calcium Mishandling, and Energetic Failure via SIRT3 Stimulation in Pulmonary Arterial Hypertension

Figure 1

Characterization of cell contraction and Ca²⁺ dynamics in isolated RV myocytes. (a) Representative profile of cellular shortening. Average of time to peak shortening (b) (CTRL: cells; PAH: cells; PAH+RES: ) and time to 50% relaxation (c) (CTRL: cells; PAH: cells; PAH+RES: ). (d) Representative florescence profile of Ca²⁺ transient. Pooled data from Ca²⁺ transient amplitude (e) and (f) (CTRL: cells; PAH: cells; PAH+RES: cells). (g) Representative images and pooled data of western blot to the SERCA2/PLB ratio; GAPDH was used as a loading control (). (h) Representative line scan images of treated groups; surface plots from selected sparks (doted square) are above; shown below are line profiles from 2 μm regions of the selected spark (black marks in line scan images). Pooled data of Ca²⁺ spark frequency (i) and amplitude (j) (CTRL: cells, 3 animals; PAH: cells, 3 animals; PAH+RES: cells, 5 animals). CTRL (solid black line), pulmonary arterial hypertension (PAH, dotted line), and PAH treated with resveratrol (PAH+RES, solid gray line). CTRL: control; PAH: pulmonary arterial hypertension; PAH+RES: PAH treated with resveratrol. All data are presented as the . vs. CTRL; ^a vs. PAH, calculated by 1-way ANOVA; ^c vs. CTRL, calculated by a 2-tailed -test.

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