Resveratrol Prevents Right Ventricle Dysfunction, Calcium Mishandling, and Energetic Failure via SIRT3 Stimulation in Pulmonary Arterial Hypertension

Figure 4

Protein acetylation and SIRT3 expression in RV. Representative image and pooled data of western blot to acetylated lysine (Ac-Lys) profile from isolated mitochondria (a) (CTRL: , PAH: , PAH+RES: ). vs. CTRL unpaired 2-tailed -test. (b) Representative images and pooled data of western blot from isolated mitochondria against SIRT3; cytochrome c oxidase subunit 4 (COX-4) was used as a loading control (CTRL: , PAH: , PAH+RES: ). (c) Immunoprecipitation (IP) of cyclophilin D (CypD) followed by immunoblot analysis against Ac-Lys; acetylation signal was normalized to total CypD (CTRL: , PAH: , PAH+RES: ). Control (CTRL); pulmonary arterial hypertension (PAH); PAH treated with resveratrol (PAH+RES). All data are presented as the . vs. CTRL, ^a vs. PAH, calculated by 1-way ANOVA.

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