The DNA Repair Enzyme XPD Is Partially Regulated by PI3K/AKT Signaling in the Context of Bupivacaine-Mediated Neuronal DNA Damage

Figure 6

Downregulation of XPD expression did not affect the PI3K/AKT pathway. In SH-SY5Y cells, bupivacaine increased the expression of XPD ((a, b) ; and ^& vs. the control group). The expression of XPD was downregulated by the XPD-GV211-RNAi-expressing lentivirus, and GV211-NC served as a control lentivirus ((a, b) ; ^#). p-PI3K and p-AKT expression was inhibited by bupivacaine ((a, c) and ^#; (a, d) and ^# vs. the control group). Nevertheless, the expression of p-PI3K ((a, c) ; ) and p-AKT ((a, d) ; ) was not further reduced in the group in which XPD expression was downregulated compared with the GV-211-NC-treated group. The data are shown as the of three independent experiments.

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