The DNA Repair Enzyme XPD Is Partially Regulated by PI3K/AKT Signaling in the Context of Bupivacaine-Mediated Neuronal DNA Damage
Downregulation of XPD expression did not affect the PI3K/AKT pathway. In SH-SY5Y cells, bupivacaine increased the expression of XPD ((a, b) ; and & vs. the control group). The expression of XPD was downregulated by the XPD-GV211-RNAi-expressing lentivirus, and GV211-NC served as a control lentivirus ((a, b) ;#). p-PI3K and p-AKT expression was inhibited by bupivacaine ((a, c) and #; (a, d) and # vs. the control group). Nevertheless, the expression of p-PI3K ((a, c) ;) and p-AKT ((a, d) ;) was not further reduced in the group in which XPD expression was downregulated compared with the GV-211-NC-treated group. The data are shown as the of three independent experiments.
Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.