Hydrogen Sulfide Attenuates Angiotensin II-Induced Cardiac Fibroblast Proliferation and Transverse Aortic Constriction-Induced Myocardial Fibrosis through Oxidative Stress Inhibition via Sirtuin 3

Figure 1

NaHS inhibits cell proliferation but enhances SIRT3 transcription in Ang II-stimulated cardiac fibroblasts. After pretreatment with NaHS (50 μM) for 4 h, neonatal rat cardiac fibroblasts were stimulated by Ang II (100 nM) for 24 h. (a) The number of cardiac fibroblasts was detected with CCK-8. (b) The content of hydroxyproline in the cell culture medium was measured. (c) Expression of SIR2 family (SIRT1-7) mRNA was measured by real-time PCR. (d) After the SIRT3 promoter luciferase plasmid was transfected into cardiac fibroblasts for 24 h, cells were pretreated with NaHS (50 μM) for 4 h followed by Ang II (100 nM) stimulation for another 24 h. Relative promoter activity of SIRT3 was detected with a dual-luciferase reporter assay system. as compared with untreated cells; ^## as compared with Ang II alone-stimulated cells. .

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