Hydrogen Sulfide Attenuates Angiotensin II-Induced Cardiac Fibroblast Proliferation and Transverse Aortic Constriction-Induced Myocardial Fibrosis through Oxidative Stress Inhibition via Sirtuin 3

Figure 2

NaHS inhibits Ang II-stimulated cardiac fibroblast proliferation via SIRT3. (a, b) After SIRT3 siRNA or NC siRNA was transfected into neonatal rat myocardial fibroblasts for 48 h, expression of SIRT3 mRNA and protein was measured by real-time PCR and western blot, respectively. as compared with cells with NC siRNA transfection. . (c) After SIRT3 siRNA or NC siRNA was transfected into cardiac fibroblasts for 24 h, the cells were pretreated with NaHS (50 μM) for 4 h followed by Ang II (100 nM) stimulation for another 24 h. The number of cardiac fibroblasts was detected with CCK-8. (d) The content of hydroxyproline in the cell culture medium was measured. as compared with untreated cells with NC siRNA transfection; ^## as compared with Ang II alone-stimulated cells with NC siRNA transfection; ^&& as compared with untreated cells with SIRT3 siRNA transfection. .

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