Hydrogen Sulfide Attenuates Angiotensin II-Induced Cardiac Fibroblast Proliferation and Transverse Aortic Constriction-Induced Myocardial Fibrosis through Oxidative Stress Inhibition via Sirtuin 3

Figure 3

NaHS suppresses collagen expression in Ang II-stimulated cardiac fibroblasts via SIRT3. After SIRT3 siRNA or NC siRNA was transfected into neonatal rat cardiac fibroblasts for 24 h, the cells were pretreated with NaHS (50 μM) for 4 h followed by Ang II (100 nM) stimulation for another 24 h. (a, b) Expression of collagen I mRNA and protein was measured by real-time PCR and western blot, respectively. (c, d) Expression of collagen III mRNA and protein was measured by real-time PCR and western blot, respectively. and as compared with untreated cells with NC siRNA transfection; ^# and ^## as compared with Ang II alone-stimulated cells with NC siRNA transfection; ^&& as compared with untreated cells with SIRT3 siRNA transfection. .

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