Hydrogen Sulfide Attenuates Angiotensin II-Induced Cardiac Fibroblast Proliferation and Transverse Aortic Constriction-Induced Myocardial Fibrosis through Oxidative Stress Inhibition via Sirtuin 3

Figure 5

NaHS decreases blood pressure but restores H₂S levels and SIRT3 expression in mice with TAC. After intraperitoneal injection by NaHS (50 μmol·kg^-1·day^-1) or normal saline (NS) for 2 weeks, male wild-type (WT) mice and SIRT3 knockout (SIRT3 KO) mice were subjected to transverse aortic constriction (TAC) surgery. NaHS or NS was administrated for another 2 weeks. (a) The level of SBP in WT mice and SIRT3 KO mice was monitored every week after NaHS administration. (b) H₂S concentration in the plasma was measured. (c) H₂S production in the myocardium was detected. (d) Expression of SIRT3 protein in the myocardium was measured by western blot. as compared with WT+Sham; ^# and ^## as compared with WT+TAC; ^&& as compared with SIRT3 KO+Sham; ^$$ as compared with SIRT3 KO+TAC. .

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