Gasotransmitter CO Attenuates Bleomycin-Induced Fibroblast Senescence via Induction of Stress Granule Formation

Figure 1

CORM-A1 inhibits BLM-induced cellular senescence in human diploid lung fibroblasts. (a) WI-38 cells were treated with various concentrations of CORM-A1 (0, 10, 20, 40, and 80 μM) for 6 h, and MTT assay was performed to assess the cell viability. (b–e) WI-38 cells were pretreated with CORM-A1 (0, 20, 40, and 80 μM) and iCORM-A1 (40 μM) for 6 h followed by the challenge of bleomycin (BLM, 25 μg/ml) for 96 h. During the process of senescence, cells were posttreated with CORM-A1 (0, 20, 40, and 80 μM) for 6 h every other day. After a 4-day incubation, the mRNA expressions of p21 (b), IL-6 (c), TNF-α (d), and IL-1β (e) were measured by quantitative real-time- (qRT-) PCR. Quantitative data are expressed as the ; . vs. the vesicle control group. ^#, ^##, and ^### vs. the BLM-alone treatment group. Then, senescence-associated- (SA-) β-gal staining (f) and immunofluorescence for detecting γ-H2AX foci (g) were applied in the pre- and posttreatment of CORM-A1 (40 μM). Quantitative data are expressed as the ( determined in three independent experiments). and . ns: not significant.

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