Gasotransmitter CO Attenuates Bleomycin-Induced Fibroblast Senescence via Induction of Stress Granule Formation

Figure 3

PAI-1 is involved in BLM-induced fibroblast senescence. (a) WI-38 cells were treated with BLM (25 μg/ml) for 96 h, and the mRNA expression of PAI-1 was detected by qRT-PCR. (b) WI-38 cells were transfected with scramble siRNA (scRNA) and siRNA against PAI-1 (siPAI-1) for 36 h, and then, PAI-1 mRNA level was assessed by qRT-PCR. Transfected cells were treated with BLM (25 μg/ml) for 96 h, and cells were performed SA-β-gal staining (c), and the mRNA expressions of p21 (d), IL-6 (e), TNF-α (f), and IL-1β (g) were measured by qRT-PCR. WI-38 cells were cotreated with PAI-1 inhibitor, TM5441 (10 μM), and BLM (25 μg/ml) for 96 h. Then, SA-β-gal staining (h) and the mRNA expressions of p21 (i), IL-6 (j), TNF-α (k), and IL-1β (l) were measured by qRT-PCR. Quantitative data are expressed as the ( determined in three independent experiments). , , and .

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