DNA-Aptamer Raised against Receptor for Advanced Glycation End Products Improves Survival Rate in Septic Mice

Figure 4

Effects of RAGE-aptamer on LPS-exposed THP-1 cells. (a–d) Each left panel shows the protein levels of TNF-α (a), IL-1β (b), IL-6 (c), and HMGB1 (d) produced by THP-1 cells. Each right panel shows the mRNA levels of cytokines in THP-1 cells. (e) ROS generation. (f) NADPH oxidase-derived superoxide generation. (g–j) mRNA levels of p22phox (g), Nox2 (h), p47phox (i), and p67phox (j). (k) Total NF-κB p65 (left panel) and p-NF-κB p65 levels (right panel) in THP-1 cells. (l) Viable cell number of THP-1 cells. per group. ^# and ^## compared with control, respectively. and compared with LPS+control-aptamer-treated cells, respectively. ^† and ^†† compared with LPS+vehicle-treated cells, respectively.

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