Ghrelin Alleviates Endoplasmic Reticulum Stress in MC3T3E1 Cells by Inhibiting AMPK Phosphorylation

Figure 5

Determination of the intracellular ROS content in different groups related to TM by DCFH-DA. (a)–(d) represent the reactive oxygen flow diagrams of the TM, , , and groups, respectively. (e) Histogram of the average fluorescence intensity of ROS in each group. GSK621 (10 μmol/L) was pretreated for 3 h, and then, ghrelin (10^-7 mol/L) was pretreated for 4 h, followed by TM (1.5 μg/mL) treatment for 20 hours. Data represent the obtained from three separate experiments. indicates comparison with the TM group, indicates , ^# indicates comparison with the group, indicates .

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