TRPV1 Channel Activated by the PGE2/EP4 Pathway Mediates Spinal Hypersensitivity in a Mouse Model of Vertebral Endplate Degeneration

Figure 1

Sensory innervation in the porous endplate in LSI mice. (a) Representative images of safranin O and fast green staining of the proteoglycan (red) and bone marrow cavities (green) in the L4-L5 caudal endplates (coronal view) in the LSI or sham group. (b) Representative images of μCT of the L4-L5 caudal endplates (coronal view) in the LSI or sham group. (c) Quantitative analysis of the percentage of endplate porosity examined by μCT. (d) Representative images of immunostaining of CGRP (red) and DAPI (blue) in the L4-L5 caudal endplates (coronal view) in the LSI or sham group. (e) Quantitative analysis of the percentage of CGRP⁺ area in the L4-L5 caudal endplates. Scale bars, 50 μm (a, d). Scale bars, 1 mm (b). vs. sham group at the corresponding time points. per group (c, e).