TRPV1 Channel Activated by the PGE2/EP4 Pathway Mediates Spinal Hypersensitivity in a Mouse Model of Vertebral Endplate Degeneration

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td class="align_left">Target gene</td><td class="align_center">Forward primer</td><td class="align_center">Reverse primer</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td class="align_left">COX2</td><td class="align_center">CAGACAACATAAACTGCGCCTT</td><td class="align_center">GATACACCTCTCCACCAATGACC</td></tr><tr><td class="align_left">PGES</td><td class="align_center">TTTCTGCTCTGCAGCACACT</td><td class="align_center">GATTGTCTCCATGTCGTTGC</td></tr><tr><td class="align_left">EP1</td><td class="align_center">GACGATTCCGAAAGACCGCAG</td><td class="align_center">CAACACCACCAACACCAGCAG</td></tr><tr><td class="align_left">EP2</td><td class="align_center">GATGGCAGAGGAGACGGAC</td><td class="align_center">ACTGGCACTGGACTGGGTAGA</td></tr><tr><td class="align_left">EP3</td><td class="align_center">TGCTGGCTCTGGTGGTGAC</td><td class="align_center">ACTCCTTCTCCTTTCCCATCTGTG</td></tr><tr><td class="align_left">EP4</td><td class="align_center">CTGGTGGTGCTCATCTGCTC</td><td class="align_center">AGGTGGTGTCTGCTTGGGTC</td></tr><tr><td class="align_left">GAPDH</td><td class="align_center">AATGTGTCCGTCGTGGATCTGA</td><td class="align_center">AGTGTAGCCCAAGATGCCCTTC</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>The primer sequence for qRT-PCR.</div>

Oxidative Medicine and Cellular Longevity

tab1

Table 1

Table 1: TRPV1 Channel Activated by the PGE2/EP4 Pathway Mediates Spinal Hypersensitivity in a Mouse Model of Vertebral Endplate Degeneration 