Neuroprotective Effects of Palmatine via the Enhancement of Antioxidant Defense and Small Heat Shock Protein Expression in Aβ-Transgenic Caenorhabditis elegans

Figure 5

The effects of palmatine on sod-3p::GFP expression, SOD, and CAT activities. (a, b) The images of sod-3p::GFP treated with or without palmatine (0.2 mM). (c) Quantification of sod-3p::GFP fluorescence intensity. The transgenic CF1553 strain was cultured at 20°C for two days on NGM plates with or without palmatine (0.2 mM). The images were obtained by using a Mshot MF52 inverted fluorescence microscope. Quantified sod-3p::GFP intensity was done by ImageJ software. The result was displayed as (–25), and the statistical analysis was carried out with unpaired -test. . (d) The effect of palmatine on the enzyme activity of SOD in wild-type worms. (e) The effect of palmatine on the enzyme activity of CAT in wild-type worms. Wild-type worms were cultured from L1- to L4-stage at 20°C on NGM plates with or without palmatine (0.2 mM), and then, the worms were lysed to detect the enzyme activities of SOD and CAT. The result was displayed as , and the statistical analysis was done by unpaired -test. ; .

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