Neuroprotective Effects of Palmatine via the Enhancement of Antioxidant Defense and Small Heat Shock Protein Expression in Aβ-Transgenic Caenorhabditis elegans

Figure 6

The effects of palmatine on hsp-16.2p::GFP expression and HSF-1 signaling pathway. (a, b) The images of hsp-16.2p::GFP in control and palmatine-treated groups. (c) Quantification of hsp-16.2p::GFP fluorescence intensity. The transgenic CL2070 strain was cultured at 20°C for two days on NGM plates with or without palmatine (0.2 mM). The images were obtained by using a Mshot MF52 inverted fluorescence microscope. Quantified hsp-16.2p::GFP intensity was done by ImageJ software. The result was displayed as (–25), and the statistical analysis was carried out with unpaired -test. . (d) RNAi analysis of the change in inhibition of Aβ-toxicity by palmatine. L1-stage CL2006 strain was treated with or without palmatine (0.2 mM) at 15°C on NGM plates containing HT115 with L4440 empty plasmid or hsf-1 recombinant plasmid for 45 h and upshifted to 20°C for inducing Aβ peptide expression. The fraction of paralytic worm was counted every day until all were palsied. The result was displayed as the Kaplan-Meier survival curve and analyzed by using a log-rank test.

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