Neuroprotective Effects of Palmatine via the Enhancement of Antioxidant Defense and Small Heat Shock Protein Expression in Aβ-Transgenic Caenorhabditis elegans

Figure 8

The effects of palmatine on fat accumulation. (a, b) The optical images upon Oil Red O staining in wild-type worms treated with or without palmatine (0.2 mM). (c, d) The optical images upon Oil Red O staining in CL4176 worms treated with or without palmatine (0.2 mM). (e) Comparative analysis of the Oil Red O intensity in wild-type and CL4176 worms treated with or without palmatine (0.2 mM). (f) Quantitative analysis of the Oil Red O intensity in wild-type worms treated with or without palmatine (0.2 mM). (g) Quantitative analysis of the Oil Red O intensity in CL4176 worms treated with or without palmatine (0.2 mM). L1-stage wild-type strain was cultured at 20°C for three days on NGM plates with or without palmatine (0.2 mM). Meanwhile, L1-stage CL4176 strain was cultured at 15°C for 36 h on NGM plates containing with or without palmatine (0.2 mM) and then placed at 23°C for another 36 h. The wild-type and CL4176 worms were collected and used for Oil Red O staining, respectively. The images were obtained by using a Mshot MF52 inverted fluorescence microscope. Quantified intensities were performed using ImageJ software. The results were displayed as (), and the statistical analyses were done by using an unpaired -test. ; .

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