Neuroprotective Effects of Palmatine via the Enhancement of Antioxidant Defense and Small Heat Shock Protein Expression in Aβ-Transgenic Caenorhabditis elegans

Table 1

The primer sequences for real-time PCR and RNAi analyses.

Gene	Forward primer (5 to 3)	Reverse primer (5 to 3)	Application
β-Actin	CCACGAGACTTCTTACAACTCCATC	CTTCATGGTTGATGGGGCAAGAG	Real-time PCR
sod-3	GAGCTGATGGACACTATTAAGCG	GCACAGGTGGCGATCTTCAAG	Real-time PCR
hsp-16.11	CTCCATCTGAATCTTCTGAGATTG	CTTCGGGTAGAAGAATAACACGAG	Real-time PCR
hsp-16.2	CTCCATCTGAGTCTTCTGAGATTGT	CTCCTTGGATTGATAGCGTACGA	Real-time PCR
hsp-16.49	TCCGACAATATTGGAGAGATTG	GATCGTTTCGAGTATCCATGCT	Real-time PCR
fat-5	GTGCTGATGTTCCAGAGGAAGAAC	ATGTAGCGTGGAGGGTGAAGCA	Real-time PCR
Fat-7	CCAGAGAAAGCACTATTTCCCAC	CACCAAGTGGCGTGAAGTGT	Real-time PCR
hsf-1^a	TGCTCTAGACTGTCCCAAGGTGGTCTAACTC	CGGGGTACCTCCCGAATAGTCTTGTTGC	RNAi

Underlines indicate the restriction sites of XbaI (TCTAGA) and KpnI (GGTACC).