BMSC-Derived Exosomes Ameliorate LPS-Induced Acute Lung Injury by miR-384-5p-Controlled Alveolar Macrophage Autophagy

Figure 4

Exosomes relieved LPS-induced autophagy stress of alveolar macrophages. (a) Image of autophagic vacuole in alveolar macrophages taken by a transmission electron microscope (TEM). The yellow arrow indicates typical autophagic vacuoles (×30,000 magnification). (b) Alveolar macrophages were transfected with an adenovirus expressing mRFP-GFP-LC3. Cells were treated with exosomes or exosome-depleted conditioned media and exposed to 10 μg/ml LPS for 24 hours. Yellow puncta (overlay) represent autophagosomes, whereas red puncta indicate autolysosomes (×400 magnification). (c) The numbers of autophagosomes and autolysosomes were determined by counting 50 cells. Values obtained from three independent experiments are expressed as . vs. the control group; vs. the control group; ^# vs. the LPS group; ^## vs. the .

(a)

(b)

(c)