Activation of the M3AChR and Notch1/HSF1 Signaling Pathway by Choline Alleviates Angiotensin II-Induced Cardiomyocyte Apoptosis

Figure 3

Choline improved Notch1 intracellular domain (NICD) expression and promoted nuclear translocation of heat shock transcription factor 1 (HSF1). (a–c) NICD protein levels (red) in NRVMs were quantified via immunostaining with the indicated antibodies; nuclei were stained with DAPI (blue). Scale bar: 50 μm. Data are presented as ; independent experiments. (d) HSF1 immunofluorescence staining. Scale bar: 50 μm. independent experiments. (e, f) Representative images and graph of HSF1 protein levels in the nuclear fraction. Data are presented as ( independent experiments). and versus control (CTR); ^# and ^### versus Ang II; ^&& and ^&&& versus the choline-treated Ang II group.

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