Research Article
Activation of the M3AChR and Notch1/HSF1 Signaling Pathway by Choline Alleviates Angiotensin II-Induced Cardiomyocyte Apoptosis
Figure 5
M3AChR siRNA blocked choline-mediated activation of the Notch1/HSF1 pathway. (a–c) The protein levels of NICD (red) in NRVMs were quantified via immunostaining with the indicated antibodies; nuclei were stained with DAPI (blue). Scale bar: 50 μm; independent experiments. (d) HSF1 immunofluorescence. Results represent five independent experiments. Scale bar: 50 μm. (e, f) Representative images and graph of HSF1 protein levels in the nuclear fraction. Data are presented as ( independent experiments). versus the negative control (NC) siRNA group; ### versus the choline-treated NC siRNA group.
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