Activation of the M3AChR and Notch1/HSF1 Signaling Pathway by Choline Alleviates Angiotensin II-Induced Cardiomyocyte Apoptosis

Figure 6

Downregulation of Notch1 blocked the protective effect of choline on Ang II-induced apoptosis. (a) Representative image and graph of Bcl-2, Bax, and cleaved-caspase-3 protein levels. (b, c) Quantification of Bcl-2/Bax and cleaved-caspase-3. Data are presented as ( independent experiments). (d) Representative image and graph of Omi/HtrA2, Smac/DIABLO, and cytochrome c protein levels in the cytosol fraction. Data are presented as ( independent experiments). (e) Representative western blot images and data of Omi/HtrA2, Smac/DIABLO, and cytochrome c protein levels in the mitochondrial fraction. Data are presented as ( independent experiments). , , and versus the NC siRNA group; ^#, ^##, and ^### versus the choline-treated negative control (NC) siRNA group.

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