Activation of the M3AChR and Notch1/HSF1 Signaling Pathway by Choline Alleviates Angiotensin II-Induced Cardiomyocyte Apoptosis

Figure 8

Choline inhibited cardiac remodeling and apoptosis in Ang II-treated rats. (a) Representative left ventricular sections were stained with H&E and Masson’s trichrome to assess cardiomyocyte hypertrophy and fibrosis, respectively; scale  μm. (b) Cross section of cardiac myocytes in the left ventricle; rats for each group with three random fields. (c) The level of fibrosis (%) stained by Masson’s trichrome; rats for each group. (d) Representative image and graph of Bcl-2, Bax, and cleaved-caspase-3 protein levels. (e, f) Quantification of Bcl-2/Bax and cleaved-caspase-3; rats for each group. (g) Representative image and graph of NICD and nuclear HSF1 protein levels. (h, i) Quantification of NICD and nuclear HSF1. Data are presented as . and versus sham; ^## and ^### versus the Ang II group.

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