LYAR Promotes Colorectal Cancer Progression by Upregulating FSCN1 Expression and Fatty Acid Metabolism

Figure 2

LYAR promotes metastasis of CRC cells. (a) Quantitative RT-PCR analysis of LYAR expression normalized to GAPDH expression in LYAR knockdown HCT15 cells. Results shown are the (). compared with the nonspecific siRNA control. (b) Western blot assay showing LYAR protein expression in LYAR knockdown HCT15 cells. GAPDH served as the loading control. (c) At left, representative photos of haptotactic migration assay and matrigel chemoinvasion assay using LYAR knockdown HCT15 cells. Original magnification, 200x. At right, the percentage of migrated and invaded LYAR knockdown HCT15 cells compared to the control. . (d) Representative images of metastastic nodules (indicated with arrows) in lung tissues after Bouin’s fixation (upper four panels) and hematoxylin and eosin (H&E) staining of tissue sections (lower two panels). (e) Visible lung metastasis counts. . (f) Visible liver metastasis counts. .

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