LYAR Promotes Colorectal Cancer Progression by Upregulating FSCN1 Expression and Fatty Acid Metabolism

Figure 5

FSCN1 overexpression restores the metastatic potential of LYAR knockdown cells. (a) Western blot assay showing LYAR and FSCN1 protein expression in LYAR knockdown (KD) HCT15 cells with and without FSCN1 overexpression (OE). GAPDH served as the loading control. (b) Representative photos of haptotactic migration assay and matrigel chemoinvasion assay using LYAR knockdown HCT15 cells with and without FSCN1 overexpression. Original magnification, 200x. (c) Results of the migration and invasion assays shown in (b). Data are shown as the (). and compared with the empty vector control. (d) Xenografted tumors of negative control (NC) cells and stable FSCN1-KD HCT15 cells (n = 8). (e) Tumor volumes over time. (f) Xenografted tumors were resected and weighed at the end of the experiment. Results are shown as the (). , , and . OE: stable FSCN1 overexpression in LYAR knockdown (KD) HCT15 cells.

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