Protection against Doxorubicin-Related Cardiotoxicity by Jaceosidin Involves the Sirt1 Signaling Pathway

Figure 5

Jaceosidin (4 mg/kg) suppressed inflammation and cardiac apoptosis in doxorubicin- (DOX-) treated mice. (a, b) P65 and IκB kinase β (IKKβ) expression (). (c) mRNA levels of inflammatory factors in the hearts (). (d) Cytokine levels in the hearts (). (e) TUNEL staining in DOX-treated mice (). (f) Bax and Bcl-2 protein expression in DOX-treated mice (). (g) Caspase-3 activity in the hearts (). Mice were intraperitoneally injected with a single dose of DOX (15 mg/kg) to establish the acute cardiac injury model. Five days after DOX injection, heart tissues were collected to assess the myocardial inflammatory response and apoptosis, as reflected in (a–g). Data are shown as . Comparisons between multiple groups were performed using one-way ANOVA followed by a post hoc Bonferroni comparison analysis. compared with saline. compared with DOX alone.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)