Protection against Doxorubicin-Related Cardiotoxicity by Jaceosidin Involves the Sirt1 Signaling Pathway

Figure 6

Jaceosidin (4 mg/kg) did not provide cardiac protection in sirtuin1- (Sirt1-) deficient cells. (a) Sirt1 protein expression in DOX-treated mice (). (b) Sirt1 protein expression in DOX-treated cells (). (c) Sirt1 activity in DOX-treated cells (). (d) Sirt1 protein expression in DOX-treated cells (). (e, f) ROS production and MDA content in DOX-treated cells (). (g) TNF-α mRNA level in DOX-treated cells (). (h) Cell viability after Sirt1 deficiency in DOX-treated cells (). For (b, c) and (e–h), cells were pretreated with jaceosidin (15 μmol/l) 6 hours before DOX (1 μmol/L) administration. To knock down Sirt1 in cardiomyocytes, NRCMs were preincubated with siSirt1 (50 nmol/l) or siRNA (50 nmol/l) for 24 hours. Data are shown as . For (d), comparisons were performed using two-tailed Student’s tests. For others, comparisons were performed using one-way ANOVA followed by a post hoc Bonferroni comparison analysis. versus the matched control.

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