Research Article
Recombinant High-Mobility Group Box 1 (rHMGB1) Promotes NRF2-Independent Mitochondrial Fusion through CXCR4/PSMB5-Mediated Drp1 Degradation in Endothelial Cells
Figure 1
rHMGB1 incubation promoted mitochondrial fusion in EA.hy926 endothelial cells. (a) The representative TEM images of mitochondria of EA.hy926 endothelial cells treated with different concentrations (control, 0.1 μg/ml, 0.5 μg/ml, and 1 μg/ml) of rHMGB1. The magnification is 5900. Scale bar: 1 μm. (b) The mitochondrial number changes under TEM in EA.hy926 endothelial cells treated with different concentrations (control, 0.1 μg/ml, 0.5 μg/ml, and 1 μg/ml) of rHMGB1. (c) The average mitochondrial area (μm2) under TEM of EA.hy926 endothelial cells treated with different concentrations (control, 0.1 μg/ml, 0.5 μg/ml, and 1 μg/ml) of rHMGB1. For comparison of average mitochondrial area, at least 30 mitochondria of 10 cells per group were calculated; for comparison of mitochondrial number, at least 10 cells per group were counted. (d) EA.hy926 endothelial cells were stained with mitochondria-specific fluorescent dye MitoTracker Green FM and imaged under a Leica SP8 confocal microscope. The magnification is 630. Scale bar (original): 50 μm; scale bar (zoom): 10 μm. TEM: transmission electron microscopy. versus control group; versus control group; versus control group; versus control group.
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