Recombinant High-Mobility Group Box 1 (rHMGB1) Promotes NRF2-Independent Mitochondrial Fusion through CXCR4/PSMB5-Mediated Drp1 Degradation in Endothelial Cells

Figure 3

Incubation with rHMGB1 reduced the protein expression of Drp1. (a) Representative immunoblotting bands of Drp1 and the matching internal standard GAPDH. (b) The densitometric analysis of relative Drp1 expression referenced to GAPDH. (c) Representative immunoblotting bands of p-Drp1-S616 and the matching internal standard GAPDH. (d) The densitometric analysis of relative p-Drp1-S616 expression referenced to GAPDH. (e) Representative immunoblotting bands of p-Drp1-S637 and the matching internal standard GAPDH. (f) The densitometric analysis of relative p-Drp1-S637 expression referenced to GAPDH. (g) rHMGB1 had no significant effect on the expression of Drp1 mRNA expression. The amount of target mRNAs was normalized to respective internal standard GAPDH mRNA; relative fold was calculated based on the ratio of the normalized values of the cells treated with rHMGB1 to that of controls (2^-ΔΔCt). GAPDH: glyceraldehyde-3-phosphate dehydrogenase. Data were expressed as the ; versus control group and versus control group.

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