Intervertebral Disc Degeneration and Low Back Pain Depends on Duration and Magnitude of Axial Compression

Figure 3

Radiological and histological evaluation of IDD with gradient compression magnitude. (a) X-ray examination showed a gradual narrowing of the intervertebral height in accordance with the severity level of compression pressure. Arrows indicated the targeted IVDs. (b) H-E staining also suggested a gradual severer IDD along with severer compression pressure. (c) Western blot analysis suggested a gradient compression magnitude induced by a gradual decrease of collagen II and aggrecan and increase of IL-1β and MMP3. (d) IHC staining indicated the distribution and content of collagen II and aggrecan decreased along with gradient compression magnitude (the data are shown as . -5 for each group. , , and when compared among each group. One-way ANOVA and Tukey’s HSD multiple comparison test were used for statistical analysis).

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