ROS Promote Hypoxia-Induced Keratinocyte Epithelial-Mesenchymal Transition by Inducing SOX2 Expression and Subsequent Activation of Wnt/β-Catenin

Figure 2

ROS are required for hypoxia-stimulated EMT and migration of HaCaT cells. (a–c) Levels of endogenous ROS in HaCaT cells were measured by staining with DCFH-DA. Fluorescent DCFH-DA was monitored at the indicated time points by a microplate reader (a, c). Representative pictures showing staining with DCFH-DA (b). (d, e) Western blotting (d) and quantitative analysis (e) were performed to detect vimentin, N-cadherin, and E-cadherin levels with or without hypoxia and NAC treatment for 12 h. β-Actin was used as the loading control. (f) Representative fluorescent images of N-cadherin and E-cadherin in the indicated HaCaT cells. Bar, 50 μm. (g, h) Scratch assays (g) and quantitative analysis (h) were performed using NAC-treated and untreated HaCaT cells with or without hypoxia exposure for 12 h. Bar, 200 μm. . . ; .

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