Neuroprotective Mechanism of Icariin on Hypoxic Ischemic Brain Damage in Neonatal Mice

Figure 12

The ERα inhibitor MPP inhibits the expression of apoptosis-related proteins in HIBD neonatal mice pretreated with ICA. The expression levels of (a) p53, (b) PUMA, (c) Bax, (d) Bcl-2, (e) caspase-3, and (f) cleaved caspase-3 in the cerebral ischemic penumbra (small black box in the upper left brain section) in MPP-treated HIBD newborn mice detected by tissue immunofluorescence, mice in each group. . (g) Representative western blot images and (h–l) quantitative analysis. (m) Quantitative analysis of the detected caspase-3 activity. , , and compared to the HI + ICA + Vehicle group. Data are presented as the , mice in each group.

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