XPO1-Mediated EIF1AX Cytoplasmic Relocation Promotes Tumor Migration and Invasion in Endometrial Carcinoma

Figure 2

EIF1AX knockdown or translocation into the nucleus inhibits proliferation, migration, and invasion of endometrial carcinoma cells in vitro. (a) CCK-8 assay was used to detect cell proliferation activity of HEC-1A (left) and RL95-2 (right) cells at 24 h, 48 h, 72 h, and 96 h. (b) The protein expression of EIF1AX, E-cadherin, vimentin, beta-catenin, and Snail following shRNA transfection. GAPDH was used as a lane loading control. (c, d) HEC-1A cells were transfected with shRNA, and the motility of the cells was evaluated 12 h after transfection using a wound-healing assay. Scale bar: 100 μm. (e–h) HEC-1A and RL95-1 cells described in (c, d) were used in a transwell migration and invasion assay. Scale bar: 100 μm. One-way ANOVA: , ns: , , and . Bars indicate SD. Note: Ctrl group (empty vector plasmid), KD group (EIF1AX-shRNA), KD + Esm group (EIF1AX-shRNA+pcDNA3.1-EIF1AXsm), and KD + NLSsm group (EIF1AX-shRNA+pcDNA3.1-EIF1AXsm-SV40NLS).

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