Research Article

Administration of N-Acetylcysteine to Regress the Fibrogenic and Proinflammatory Effects of Oxidative Stress in Hypertrophic Ligamentum Flavum Cells

Figure 2

(a) Immunofluorescence analysis of ligamentum flavum cells. Immunofluorescence was visualized using FITC (green) and the nuclei by DAPI (blue). Cells cultured in the current study expressed collagen type I, collagen type III, and fibronectin. (b) The alkaline phosphatase activity was assessed by BCIP-NBT (Sigma). The alkaline phosphatase activity was detected intracellularly in over 80% of cells, the morphology of which was primarily spindle-shaped and polygonal.
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