Anthocyanin Encapsulated Nanoparticles as a Pulmonary Delivery System

Figure 8

Detection of cyanidin-3-O-glucoside (C3G), the major anthocyanin present in HB in lung tissue of A/JCr mice which were given pulmonary delivery of nanoparticles or HB extract. Nanoparticles (E-CMC and HB-CMC) and HB extracts were dissolved separately in sterilized deionized water (4 μg C3G equivalent HB anthocyanin in 1 mL water) aerosolized by an ultrasonic mesh nebulizer to facilitate passive inhalation through a nose-only exposure device. The inhalation treatment (28 μg C3G equivalent nanoparticles/mouse/day) was given for six days, leaving two days gap between each treatment, and lung tissues were collected and analyzed by UPLC/ESI/MS-O-TOF for anthocyanin metabolites. C3G was detected after 9.2 min in HB-CMC and HB extract-treated mice lung tissue samples. Chromatograms shown are composites C3G standard (a), lung tissue from mice of control (E-CMC) (b), HB-CMC (c), and HB extract inhalation (d). E-CMC: anthocyanin-free carboxymethyl chitosan nanoparticles; HB-CMC: HB anthocyanin-encapsulated nanoparticles; HB: haskap berry anthocyanin.