1,25(OH)₂D₃ Mitigates Oxidative Stress-Induced Damage to Nucleus Pulposus-Derived Mesenchymal Stem Cells through PI3K/Akt Pathway

Figure 2

(a) 1,25(OH)₂D₃ shows no cytotoxicity effect on cell viability at a concentration of 10^-8 M to 10^-12 M for 24 h. (b) 100 μM H₂O₂ treatment for 6 h leads to appropriate inhibition of cell proliferation. (c) EdU assay results of nucleus pulposus-derived mesenchymal stem cells (NPMSCs) in the different groups. Green fluorescence represents cells in a proliferating state ( μm;  μm). (d) Quantitative analysis of EdU-staining positive cells. All data are the of at least three independent experiments. and compared with the control group; ^## compared with the H₂O₂ group; ^&& compared with the 1,25(OH)₂D₃ group. LY: LY294002 group.

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