Research Article
1,25(OH)2D3 Mitigates Oxidative Stress-Induced Damage to Nucleus Pulposus-Derived Mesenchymal Stem Cells through PI3K/Akt Pathway
Figure 3
SA-β-gal staining assay and the expression levels of p21 and p53. (a) SA-β-gal staining results of nucleus pulposus-derived mesenchymal stem cells (NPMSCs) in the different groups. Aging cells displayed high expression of SA-β-gal in blue staining ( μm; μm). (b) Quantitative analysis of SA-β-staining results. (c) Immunofluorescence staining of p21 is observed by a laser scanning confocal microscope, and the immunofluorescence staining of p53 is observed by a fluorescence microscope ( μm). (d) Quantitative analysis of the fluorescence intensity of p21 and p53. (e, f) The protein expressions and quantitative analysis of p21 and p53. All data are expressed as the . compared with the control group; ## compared with the H2O2 group; && compared with the 1,25(OH)2D3 group. LY: LY294002 group.
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