Research Article
1,25(OH)2D3 Mitigates Oxidative Stress-Induced Damage to Nucleus Pulposus-Derived Mesenchymal Stem Cells through PI3K/Akt Pathway
Figure 5
1,25(OH)2D3 upregulates the expression of Bcl-2 and downregulates the expression of Bax and Caspase-3. (a, b) Immunofluorescence staining and quantitative analysis of Bax ( μm). (c, d) Immunofluorescence staining and quantitative analysis of Bcl-2 ( μm). (e, f) Immunofluorescence staining and quantitative analysis of Caspase-3 ( μm). Immunofluorescence staining was observed by a fluorescence microscope. (g, h) The protein expressions and quantitative analysis of Bcl-2, Bax, and Caspase-3 in the different groups. (i) Quantitative analysis of the mRNA expression levels of Bcl-2, Bax, and Caspase-3. All data are the . and compared with the control group; # and ## compared with the H2O2 group; & and && compared with the 1,25(OH)2D3 group. LY: LY294002 group.
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