1,25(OH)₂D₃ Mitigates Oxidative Stress-Induced Damage to Nucleus Pulposus-Derived Mesenchymal Stem Cells through PI3K/Akt Pathway

Figure 6

1,25(OH)₂D₃ protects mitochondrial membrane potential (MMP) and reduces reactive oxygen species (ROS) in nucleus pulposus-derived mesenchymal stem cells (NPMSCs). (a) Fluorescence results of MMP in the different groups. Mitochondrial aggregate JC-1 emits red fluorescence, and monomer JC-1 emits green fluorescence ( μm;  μm). (b) Quantitative analysis of MMP fluorescence intensity. (c) Fluorescence results of ROS in the different groups. A stronger red fluorescence indicates a higher ROS level. (d) Quantitative analysis of ROS fluorescence results. All data are the . compared with the control group; ^## compared with the H₂O₂ group; ^&& compared with the 1,25(OH)₂D₃ group. LY: LY294002 group.

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