1,25(OH)₂D₃ Mitigates Oxidative Stress-Induced Damage to Nucleus Pulposus-Derived Mesenchymal Stem Cells through PI3K/Akt Pathway

Figure 7

1,25(OH)₂D₃ alleviates the inhibition of H₂O₂ on the PI3K/Akt pathway. (a) Immunofluorescence staining of Akt and p-Akt in nucleus pulposus-derived mesenchymal stem cells (NPMSCs) is observed by a laser scanning confocal microscope ( μm). (b) Quantitative analysis of the fluorescence expressions of Akt and p-Akt. (c, d) The protein expression and quantitative analysis of Akt and p-Akt in the different groups. All data are the . and compared with the control group; ^# and ^## compared with the H₂O₂ group; ^& and ^&& compared with the 1,25(OH)₂D₃ group. LY: LY294002 group.

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