The Effect of Allograft Inflammatory Factor-1 on Inflammation, Oxidative Stress, and Autophagy via miR-34a/ATG4B Pathway in Diabetic Kidney Disease

Figure 3

The effect of AIF-1 or miR-34a on autophagy and inflammation in DKD mice. (a) The expression of AIF-1 in db/db mice treatment with miR-34a agonist or antagonist was evaluated by immunohistochemical staining. (b)The effect of miR-34a on the expression of AIF-1, ATG4B, LC3II, p62, and NLRP3 protein in db/db mice was detected by western blot. Versus db/m mouse group, . (c) The expression of ATG4B, LC3II, p62, and NLRP3 protein in AIF-1 transgenic mice was detected by western blot. Versus wild mice group, . (d) The expression of miR-34a in AIF-1 transgenic mice was detected by qPCR. Versus wild mice group, . AIF-1: allograft inflammatory factor-1; miR-34a: microRNA 34a; ATG4B: autophagy related 4 homolog B; LC3II and p62: autophagy-associated protein; NLRP3: NOD-like receptor thermal protein domain associated protein 3.

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