The Effect of Allograft Inflammatory Factor-1 on Inflammation, Oxidative Stress, and Autophagy via miR-34a/ATG4B Pathway in Diabetic Kidney Disease

Figure 4

The effect of glucose on autophagy, inflammation, and ROS of HRGECs with a time- and concentration-dependent manner. (a–c) The effect of high concentration glucose (30 mmol/L) on AIF-1, miR-34a, ATG4B, LC3II, p62, NLRP3, and ROS level in HRGECs with a time-dependent manner. Versus 0 h group, . (d–f) The effect of glucose on AIF-1, miR-34a, ATG4B, LC3II, p62, NLRP3, and ROS level in HRGECs with a concentration-dependent manner for 48 h. All the relative levels of results were corrected by total protein. Versus 5.6 mmol/L glucose group, . HRGECs: human renal glomerular endothelial cells; AIF-1: allograft inflammatory factor-1; miR-34a: microRNA 34a; ATG4B: autophagy related 4 homolog B; LC3II and p62: autophagy-associated protein; NLRP3: NOD-like receptor thermal protein domain associated protein 3; ROS: reactive oxygen species.

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