The Effect of Allograft Inflammatory Factor-1 on Inflammation, Oxidative Stress, and Autophagy via miR-34a/ATG4B Pathway in Diabetic Kidney Disease

Figure 6

The effect of ATG4B on autophagy and inflammation in HRGECs cultured with high concentration glucose. (a) The relationship between ATG4B and miR-34a was analyzed via luciferase reporter assay. Versus ATG4B-MUT group, . (b) The effect of ATG4B on the level of NLRP3 and IL18 in HRGECs cultured in 30 mmol/L glucose for 48 h. Versus high glucose control group, . (c, d) The effect of ATG4B on the expression of LC3II and p62 in HRGECs cultured in 30 mmol/L glucose for 48 h. All the relative levels of results were corrected by total protein. Versus 30 mmol/L glucose control group, . HRGECs: human renal glomerular endothelial cells; miR-34a: microRNA 34a; ATG4B: autophagy related 4 homolog B; LC3II and p62: autophagy-associated protein; NLRP3: NOD-like receptor thermal protein domain associated protein 3.

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