[Retracted] Sirtuin 1 Induces Choroidal Neovascularization and Triggers Age-Related Macular Degeneration by Promoting LCN2 through SOX9 Deacetylation

Figure 2

SOX9 upregulates LCN2 expression. (a) A heat map of DEGs between 151 normal samples and 142 AMD samples in GSE29801 dataset. (b) A heat map of DEGs between 8 normal samples and 8 AMD samples in GSE103060 dataset. (c) Venn diagram of the intersection among 4 datasets. (d) The expression of SOX9 in GSE29801 dataset (151 normal samples and 142 AMD samples). (e) The expression of SOX9 in GSE103060 dataset. (f) The protein expression of SOX9 in control and hypoxia-exposed ARPE-19 cells determined by Western blot analysis; , compared with control ARPE-19 cells. (g) The protein expression of SOX9 in hypoxia-exposed ARPE-19 cells after different transfection determined by Western blot analysis. (h) Binding sites between SOX9 and LCN2 determined by ChIP-PCR; , compared with IgG. (i) The effect of SOX9 on LCN2 promoter activity in HEK-293T cells determined by dual luciferase reporter gene assay; , compared with HEK-293T cells transfected with oe-NC. (j) The protein expression of LCN2 in hypoxia-exposed ARPE-19 cells after different transfection determined by Western blot analysis. The experiment was repeated three times. , compared with hypoxia-exposed ARPE-19 transfected with sh-NC; ^#, compared with hypoxia-exposed ARPE-19 cells transfected with oe-NC. . ^###.

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