[Retracted] Sirtuin 1 Induces Choroidal Neovascularization and Triggers Age-Related Macular Degeneration by Promoting LCN2 through SOX9 Deacetylation

Figure 3

SIRT1 promotes the SOX9 nuclear translocation through the deacetylation of SOX9, thereby increasing the expression of LCN2. (a) The protein expression of SIRT1 in control and hypoxia-exposed ARPE-19 cells determined by Western blot analysis. , compared with control ARPE-19 cells. (b) The protein of SIRT1 in hypoxia-exposed ARPE-19 cells after different transfection determined by Western blot analysis. , compared with hypoxia-exposed ARPE-19 cells transfected with sh-NC. (c) The protein expression of SOX9 and acetylated SOX9 in hypoxia-exposed ARPE-19 cells after different transfection determined by Western blot analysis. , compared with hypoxia-exposed ARPE-19 cells transfected with oe-NC. ^####, compared with hypoxia-exposed ARPE-19 cells transfected with sh-NC. (d) The nuclear localization of SOX9 in hypoxia-exposed ARPE-19 cells after different transfections determined by immunofluorescence staining (scale bar: 25 μm). (e) The protein expression of SIRT, acetylated SOX9, and LCN2 in the hypoxia-exposed ARPE-19 cells after different transfections determined by Western blot analysis. (f) The content of VEGF, TNF-α, and IL-6 in the conditioned medium of hypoxia-exposed ARPE-19 cells after different transfections determined by ELISA. (g) Hypoxia-exposed ARPE-19 cell viability detected by CCK-8. (h) Hypoxia-exposed ARPE-19 cell apoptosis detected by flow cytometry. (i) HUVEC migration detected by Transwell assay. (j) HUVEC angiogenesis detected by tube formation experiment. In (e)–(h), , compared with hypoxia-exposed ARPE-19 cells transfected with sh-NC+oe-NC. ^#, compared with hypoxia-exposed ARPE-19 cells transfected with sh-SIRT1+oe-NC. In (i) and (j), , compared with HUVECs transfected with sh-NC+oe-NC; ^#, compared with HUVECs transfected with sh-SIRT1+oe-NC. . .

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