Aberrant Expression of Mitochondrial SAM Transporter SLC25A26 Impairs Oocyte Maturation and Early Development in Mice

Figure 1

Expression pattern of mRNA and protein of Slc25a26 during mouse oocyte maturation and embryo preimplantation development. (a) The mRNA levels of Slc25a26 in GV, MI, and MII oocytes and mouse embryos at zygote, 2-cell, 4-cell, 8-cell, morula, and blastocyst stages were examined by qRT-PCR. 20–30 oocytes and embryos were collected for each group. (b) GV, MI, and MII oocytes and zygote, 2-cell, 4-cell, 8-cell, morula, and blastocyst stage embryos were immunolabeled with anti-SLC25A26 antibody to identify subcellular localization and expression of SLC25A26 during oocyte maturation and preimplantation development. Red, SLC25A26; blue, DNA.  μm. (c) The protein levels of SLC25A26 in GV, MI, and MII oocytes and embryos at zygote, 2-cell, 4-cell, 8-cell, morula, and blastocyst stages were measured with ImageJ for quantification of fluorescence intensities ( for each group). A.U. means arbitrary units. Data are presented as the .

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