Aberrant Expression of Mitochondrial SAM Transporter SLC25A26 Impairs Oocyte Maturation and Early Development in Mice

Figure 2

Effects of SLC25A26 expression on the meiotic progression of mouse oocytes. (a) Representative images of SLC25A26 protein staining in young and aged oocytes. Scale  μm. (b) The fluorescence intensity of SLC25A26 signals was measured in young and aged oocytes. . A.U. means arbitrary units. (c) Representative western blotting images of SLC25A26 protein in young and aged oocytes. (d) The mean densitometric quantification of SLC25A26 protein level as measured in young and aged oocytes with ImageJ. . (e) Schematic of overexpressing the SLC25A26 in GV oocyte by microinjection and its IVM culture. Efficiency of SLC25A26 overexpression (SLC25A26-OE) after mRNA injection was verified by immunofluorescence: (f) representative images of FLAG protein staining in control and SLC25A26-OE group. Scale  μm. (g) The fluorescence intensity of FLAG signals was measured in control and SLC25A26-OE oocytes. . A.U. means arbitrary units. (h) SLC25A26 protein level was determined by western blot analysis. (i) The mean densitometric quantification of SLC25A26 protein level as measured in control and SLC25A26-OE oocytes with ImageJ. . (j) Representative images of control and SLC25A26-OE oocytes. Scale  μm. (k and l) Quantitative analysis of GVBD and Pb1 extrusion rate in control and SLC25A26-OE. and . Data are presented as the .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)