Aberrant Expression of Mitochondrial SAM Transporter SLC25A26 Impairs Oocyte Maturation and Early Development in Mice

Figure 7

Effects of SLC25A26 overexpression on preimplantation embryos and SLC25A26 knockout on postimplantation embryo development. (a) Representative images of SLC25A26 protein staining in control and SLC25A26-OE 2-cell embryos. Scale  μm. The fluorescence intensity of SLC25A26 signals was measured in control and SLC25A26-OE 2-cell embryos with ImageJ. A.U. means arbitrary units. Data are presented as the . . (b) Representative image of E4.5 control and SLC25A26-OE embryos. Scale  μm. And the blastocyst formation rate was recorded in control and SLC25A26-OE embryo after fertilization. Data are presented as the . . (c) Representative image of genotyping PCR products visualized on agarose gel. The bands indicated by arrow were amplified from wild-type and knockout allele, respectively. (d) Representative image of embryos at 7.5, 8.0, 8.5, and 9.5 dpc obtained by crossing Slc25a26 heterozygous mice. Wild-type (+/+), heterozygous (+/–), and knockout embryos are shown. Scale  μm. (e) Representative image of embryos at 10.5 dpc. Wild-type (+/+), heterozygous (+/–), and resorption embryos are shown. Scale  μm.

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