Research Article
Depleted Long Noncoding RNA GAS5 Relieves Intervertebral Disc Degeneration via microRNA-17-3p/Ang-2
Figure 2
GAS5 stimulates NPC apoptosis and inhibits cell proliferation and ECM remodeling. (a, b) Western blot analysis of ECM metabolism-related and apoptosis-related proteins in NPCs, which was normalized to GAPDH. . (c) Viability of NPCs assessed by CCK-8 assay. . (d, e) TUNEL staining analysis of NPCs (×400). vs. HDNPCs treated with oe-NC and # vs. HDNPCs treated with si-NC. The cell experiment was repeated independently 3 times. Data () were assessed by one-way ANOVA, followed by Tukey’s post hoc test. Data among groups at different time points were assessed with repeated measures ANOVA, followed by Bonferroni test. The experiment was repeated 3 times.
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