Depleted Long Noncoding RNA GAS5 Relieves Intervertebral Disc Degeneration via microRNA-17-3p/Ang-2

Figure 8

Downregulated GAS5/Ang-2 or elevated miR-17-3p rescues the inhibitory effect of IL-1β on ECM remodeling. (a) Protein levels of MMP3 in HDNPCs measured by Western blot analysis. (b) Protein levels of MMP13 in NPCs. (c) Protein levels of ADAMTS4 in HDNPCs measured by Western blot analysis. (d) Protein levels of E ADAMTS5 in HDNPCs measured by Western blot analysis. (e) Protein levels of collagen II in HDNPCs measured by Western blot analysis. (f) Protein levels of aggrecan in HDNPCs measured by Western blot analysis. vs. HDNPCs without any treatment; ^# vs. HDNPCs treated with si-NC and 10 ng/mL IL-1β; ^$ vs. HDNPCs treated with NC mimic and 10 ng/mL IL-1β. Data () were assessed by one-way ANOVA, followed by Tukey’s post hoc test. The experiment was repeated 3 times.

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